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hur overexpression plasmid  (Shanghai GenePharma)

 
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    Shanghai GenePharma hur overexpression plasmid
    Hur Overexpression Plasmid, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hur overexpression plasmid/product/Shanghai GenePharma
    Average 90 stars, based on 1 article reviews
    hur overexpression plasmid - by Bioz Stars, 2026-02
    90/100 stars

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    HUR regulated MAPKAPK3 expression and cell proliferation and mobility . U251 and U87 cells were treated with the NC siRNA + vector, HUR siRNA + vector, and HUR siRNA + MAPKAPK3 overexpression plasmid (MAPKAPK3-OE). (a–b) Western blotting was used to detect the protein expression of HUR, MAPKAPK3, p-MAPKAPK3, and p-CREB in each group. (c) The CCK-8 assay was used to detect the cell viability in each group in 48 h. (d-e) Transwell assays were used to detect the invasive cell numbers in each field in each group. (f–g) Western blotting was used to detect the expression of MAPKAPK3 in TSPO knockdown cells with HUR overexpression. (h) Amanitin (0.1 µM) was used to inhibit the mRNA synthesis for 0, 2, 4, 6, and 8 h, and qRT-PCR results indicated that overexpression of HUR increased the mRNA stability of MAPKAPK3 in U87 and U251 cells with TSPO inhibition. * P < 0.05; ** P < 0.01.

    Journal: Bioengineered

    Article Title: Inhibition of translocator protein 18 kDa suppressed the progression of glioma via the ELAV-like RNA-binding protein 1/MAPK-activated protein kinase 3 axis

    doi: 10.1080/21655979.2022.2048992

    Figure Lengend Snippet: HUR regulated MAPKAPK3 expression and cell proliferation and mobility . U251 and U87 cells were treated with the NC siRNA + vector, HUR siRNA + vector, and HUR siRNA + MAPKAPK3 overexpression plasmid (MAPKAPK3-OE). (a–b) Western blotting was used to detect the protein expression of HUR, MAPKAPK3, p-MAPKAPK3, and p-CREB in each group. (c) The CCK-8 assay was used to detect the cell viability in each group in 48 h. (d-e) Transwell assays were used to detect the invasive cell numbers in each field in each group. (f–g) Western blotting was used to detect the expression of MAPKAPK3 in TSPO knockdown cells with HUR overexpression. (h) Amanitin (0.1 µM) was used to inhibit the mRNA synthesis for 0, 2, 4, 6, and 8 h, and qRT-PCR results indicated that overexpression of HUR increased the mRNA stability of MAPKAPK3 in U87 and U251 cells with TSPO inhibition. * P < 0.05; ** P < 0.01.

    Article Snippet: The plasmids overexpressing MAPKAPK3 and HUR were obtained from Juneng Huida Biological Co., Ltd. (Wuhan, China).

    Techniques: Expressing, Plasmid Preparation, Over Expression, Western Blot, CCK-8 Assay, Knockdown, Quantitative RT-PCR, Inhibition

    Overexpression of HUR or MAPKAPK3 reversed the inhibitory effects of TSPO inhibition of glioma cells . U251 and U87 cells were treated with NC siRNA, TSPO siRNA, TSPO siRNA + HUR-OE, and TSPO siRNA + MAPKAPK3-OE. (a) The CCK-8 assay was used to detect the proliferative rate of U87 and U251 cells in each group treated with the aforementioned conditions in 24 and 48 h. (b) EDU assays were used to detect the EDU-positive rate in each group. (c) Transwell assay was performed to detect the number of invasive cells in each field in each group. * P < 0.05; ** P < 0.01.

    Journal: Bioengineered

    Article Title: Inhibition of translocator protein 18 kDa suppressed the progression of glioma via the ELAV-like RNA-binding protein 1/MAPK-activated protein kinase 3 axis

    doi: 10.1080/21655979.2022.2048992

    Figure Lengend Snippet: Overexpression of HUR or MAPKAPK3 reversed the inhibitory effects of TSPO inhibition of glioma cells . U251 and U87 cells were treated with NC siRNA, TSPO siRNA, TSPO siRNA + HUR-OE, and TSPO siRNA + MAPKAPK3-OE. (a) The CCK-8 assay was used to detect the proliferative rate of U87 and U251 cells in each group treated with the aforementioned conditions in 24 and 48 h. (b) EDU assays were used to detect the EDU-positive rate in each group. (c) Transwell assay was performed to detect the number of invasive cells in each field in each group. * P < 0.05; ** P < 0.01.

    Article Snippet: The plasmids overexpressing MAPKAPK3 and HUR were obtained from Juneng Huida Biological Co., Ltd. (Wuhan, China).

    Techniques: Over Expression, Inhibition, CCK-8 Assay, Transwell Assay